Specific reduction of wheat storage proteins by thioredoxin h.
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چکیده
منابع مشابه
Specific reduction of wheat storage proteins by thioredoxin h.
Gliadins and glutenins, the major storage proteins of wheat endosperm (Triticum durum, Desf. cv Monroe), were reduced in vitro by the NADP/thioredoxin system (NADPH, NADP-thioredoxin reductase and thioredoxin; in plants, the h type) from either the same source or the bacterium Escherichia coli. A more limited reduction of certain members of these protein groups was achieved with the reduced fo...
متن کاملCloning of thioredoxin h reductase and characterization of the thioredoxin reductase-thioredoxin h system from wheat.
Thioredoxins h are ubiquitous proteins reduced by NADPH- thioredoxin reductase (NTR). They are able to reduce disulphides in target proteins. In monocots, thioredoxins h accumulate at high level in seeds and show a predominant localization in the nucleus of seed cells. These results suggest that the NTR-thioredoxin h system probably plays an important role in seed physiology. To date, the study...
متن کاملGrouping of bread wheat cultivars by seed storage proteins. Sonia Kahrizi1, Mohammad Sedghi2* and Omid Sofalian2
To determine seed storage protein banding patterns in some bread wheat cultivars and the similarity of banding patterns among different cultivars, an experiment based on seed storage protein electrophoresis (albumin and globulin) was performed. Water and salt soluble proteins were extracted in sixteen wheat cultivars using polyacrylamide gel electrophoresis and banding pattern was obtained. Stu...
متن کاملTrafficking of storage proteins in developing grain of wheat
The processing properties of the wheat flour are largely determined by the structures and interactions of the grain storage proteins (also called gluten proteins) which form a continuous visco-elastic network in dough. Wheat gluten proteins are classically divided into two groups, the monomeric gliadins and the polymeric glutenins, with the latter being further classified into low molecular wei...
متن کاملThe characterization of cDNA clones coding for wheat storage proteins.
Poly(A)+ RNA isolated from the developing wheat endosperm var. Chinese Spring, has been used as template for the construction of a cDNA library. Within the library, clones have been identified by in vitro translation of hybrid-selected mRNA which encode alpha/beta gliadin related sequences and gamma-gliadin related sequences. The DNA sequence of one such clone has been determined and it shows h...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1992
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.99.3.919